The positive Amino odour test was noticed in 45, 23% (38/84) of the test group examinees, since the control group examinees had unfavorable test (p <0, 001; 2= 61, 879). a positive amino odour test, an occurrence of clue cells in a direct microscopic concoction of vaginal discharge and assessment of the state of vaginal flora for bacterial vaginosis are significantly more frequent occurrences in women with individual discomforts. It was proved thatG. vaginalisis a dominant micro organism in 95% of women with clinical signs of vaginosis although it was isolated from vaginal discharge in 40 to 50% of healthy women. In our research, G. vaginaliswas isolated in 63, 41% of examined women with all signs of bacterial vaginosis, in 36, 59% of examined women with one or more clinical signs of bacterial vaginosis and in 2, 58% of examined women of control group without clinical signs. Keywords: bacterial vaginosis, Gardnerella vaginalis == INTRODUCTION == An increased vaginal discharge is one of the most frequent symptoms of female genital tract. The most recent research have shown Sophoridine that 96% of all increased vaginal discharges or infections result from five says: bacterial vaginosis Sophoridine from 4 to 64%; vulvovaginal candidiasis from 28 to 37%; cervicitis caused byChlamydia trachomatis, Neisseria gonorrhoea, Herpes simplexvirus type 2, from 2 to 20%; trichomonas colpitis in around 10% of women and finally superfluous but normal discharge (1). Bacterial vaginosis is a chronic or relapsing syndrome connected to unidentified brokers, and pathogenesis is polymicrobial and multicausal. It was internationally defined in 1984 as replacement of vaginalLactobacilluswith specific group of bacteria, because of which, the characteristics of vaginal discharge are changed (3). Clinical signs and symptoms of bacterial vaginosis are: an increased grey-white vaginal discharge with consistency similar to milk and with strong odour of fish. Bacterial vaginosis is associated with an increased risk from several pathological says including post surgery infections which arose after hysterectomy, postabortal pelvic inflammatory disease and plasma cell endometritis (4, 5, 6). In expectant mothers, bacterial vaginosis is associated with the presence of fetal fibronectin in cervkovaginal discharge during the second and the third trimester by which the risk of clinical chorioamnionitis, neonatal sepsis, preterm births has been increased for 16 times. One of the consequences of bacterial vaginosis might be cervical neoplasia due to oncogene potential of nitrosamine which is produced by anaerobic bacteria in causal relation to bacterial vaginosis (7). Women who have had more than five sexual partners, users of intrauterine devices, Sophoridine smokers and persons who are diagnosed with some other sexually contagious disease suffer more frequently from bacterial vaginosis (8, 9). Bacterial vaginosis arises as a result of normal vaginal flora exchange(Lactobacillus)with mixed bacterial flora which consists ofGardnerella vaginalis, anaerobic bacteria(Prevotella bivia, Prevotella disiens, Prevotella species, Peptostreptococcus spp., Mobiluncus species)andMycoplasme hominis. It has been proved thatGardnerella vaginalis (G. vaginalis)is a dominant micro organism in 95% of women with clinical signs of vaginosis although it has been isolated from vaginal discharge in 40 to 50% of healthy women. The occurrence of Mouse monoclonal to FAK frequent relapses which appears in 20 to 30% of women within 3 months after treatment is explained by reinfection with another biotype ofG. vaginaliswhich is different from genuine biotype or as a consequence of improper therapy (10). Pathogenesis of this more and more frequent clinical syndrome has not been cleared so far. The majority of authors consider hormonal factors as those which have a major role in Sophoridine pathogenesis of bacterial vaginosis, because that state hits only the women of Sophoridine reproductive age (7). According to other authors, bacterial vaginosis is the result of changed vaginal antibiosis or antagonism between micro organisms which exist in vagina. Antibiosis is under the influence of vaginal discharge pH lowered by lactobacillis production of acid products. The diagnosis of bacterial vaginosis is clinically and laboratory established. It is necessary to satisfy at least three out of four accepted criteria in order to reach a clinical diagnosis: acidity of vaginal discharge higher than 4, 5; homogeneous, adherent discharge; positive amino odour test; the presence of clue cells (11). Vaginal discharge pH is the most sensitive, but the least specific characteristic of Amsels criteria. There are many factors such as vaginal rinsing, sexual intercourse, bleeding that can influence the value of pH. Acidity of vaginal discharge lower than 4, 5 rejects the possibility of the presence of bacterial vaginosis. Vaginal discharge whose odour is unpleasant is the only clinical symptom stated by the women with bacterial vaginosis. In.